علوم زیستی دریا
Razieh Nasrolahzadeh; Alireza Safahieh; Hossein Zolgharnein; Issac Zamani; Kamal Ghanemi
Abstract
Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, ...
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Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, in which the bacterial communities plays a major role in the biodegradation of BPA in the environment.In this study, contaminated sediments were collected in Mousa Creek. The isolates were identified by biochemical tests and 16S rRNA gene sequence analysis. The isolated Bacteri was Pseudomonas putida. Growth and degradation ability of isolated baccteri was measured in 100, 200 and 300 ppm of BPA in 24-hour intervals for 6 days. Bacterial growth rate was detected with spectrophotometer at 600 nm. Efficiency of BPA degradation was performed by Liquid-liquid extraction method and measured by HPLC. This bacteri was able to grow at concentrations of 100, 200 and 300 ppm of BPA. So, the biodegradation results from mineral salt medium (MSM) indicated that Pseudomonas putida have the best degradation efficiency at the 200 ppm of the BPA. Biodegredation result by HPLC method show that the percentage of the degradation efficiency at the 200 ppm was 82.8 percent.These results demonstrated this indigenous bacteria (Pseudomonas putida) can be used to improve the bacterial communities contaminated by Bisphenol A and to eliminate the pollutants from the costal environment.